An improved and simplified somatic embryogenesis protocol in Chir pine (Pinus roxburghii).

ABSTRACT: Forests are considered to be an important pillar for a country's bioeconomy but unfortunately are being depleted globally. Pinus roxburghii has considerable ecological and economic importance for Pakistan. It is successfully used as a pioneer species in afforestation and reforestation programs in the era of climate change. This paper reports on a significantly improved protocol for P. roxburghii somatic embryogenesis. Successive developmental stages for zygotic as well as somatic embryos were reported first time for this important pine species. Initiation of somatic embryogenesis was affected by the developmental stage of the zygotic embryo. Immature pre-cotyledonary zygotic embryos produced 33% initiation of embryonic masses on LP-889 medium supplemented with 11 µM Naphthaleneacetic acid

SUBMITTER: Kousar F 

PROVIDER: S-EPMC12569014 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature