Nanotechnology Meets superbugs: biocompatible polymeric nanoparticles combat MDR Klebsiella pneumoniae via gene suppression and biofilm Inhibition.

ABSTRACT: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a critical threat in healthcare settings due to its limited treatment options and high resistance to conventional antibiotics. This study explores the potential of polycaprolactone (PCL) nanospheres as a delivery vehicle for imipenem to combat multidrug-resistant CRKP. Imipenem-loaded nanospheres, with an average size of 610 ± 125 nm and an encapsulation efficiency of 84.5%, were synthesized using a double-emulsion method and characterized for morphology, size, drug loading, and release profile. The formulation demonstrated a substantial improvement in antibacterial activity, with an eightfold reduction in minimum inhibitory concentration compared to free imipenem. The nanoparticles also significantly inhibited biofilm formation and acce

SUBMITTER: Ebrahimi FA 

PROVIDER: S-EPMC12569084 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature