Systemic hypoxia appears to attenuate autophagy in human peripheral blood mononuclear cells during repeated sprint exercises.

ABSTRACT: This research investigated the modulation of autophagy and protein synthesis markers in human peripheral blood mononuclear cells (PBMCs) of moderately trained men following repeated sprint exercises (RSE) performed under hypoxia (HYP, 13% FIO₂), with bilateral blood flow restriction (BFR, 45% of resting arterial occlusive pressure), or normoxia. Using a crossover design, participants completed three sets of five 10-s sprints under each condition. mRNA and protein levels were assessed using qPCR and Western immunoblotting. Exercise significantly increased the microtubule-associated light chain 3B (LC3B)-II/I ratio (p < 0.001, dz = 0.58), with the effect being more pronounced in NOR (p = 0.011, dz = 0.81). Conversely, p62 protein levels were unchanged (p > 0.05). However, a tenden

SUBMITTER: Solsona R 

PROVIDER: S-EPMC12569098 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature