Ultrasonic rewarming of cryopreserved alginate encapsulated liver spheroids.

ABSTRACT: Rapid volumetric rewarming methods are needed to enable the effective cryopreservation and recovery of large volumes of biological cells for therapy and banking of tissues and organs. Ultrasonic rewarming is currently under development, but its effect on cells and their post-rewarming viability has not yet been established. Here, we compare ultrasonic rewarming with the gold-standard [Formula: see text]C water bath using cryovials containing cryopreserved alginate encapsulated liver spheroids. Mean rewarming rates are used to establish the exposure time to rewarm to [Formula: see text]C for higher power (100 W) and lower power (20 W) ultrasonic rewarming. These electrical powers correspond to free-field pressures along the central cryovial axis of 2.8 MPa and 1.3 MPa, respectively. Ultraso

SUBMITTER: Xu R 

PROVIDER: S-EPMC12569155 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature