Molecular and pathological insights into gene expression and oxidative stress in Clinostomum complanatum and Euclinostomum heterostomum.

ABSTRACT: Parasitic infections caused by Clinostomum complanatum and Euclinostomum heterostomum pose significant threats to aquaculture systems and public health. This study examined their molecular, immunological, and pathological impacts in Egyptian Nile tilapia (Oreochromis niloticus), with COXI gene sequencing confirming both species and revealing genetic similarities to regional isolates, suggesting broad geographical distribution. Euclinostomum heterostomum-infected fish exhibited significant upregulation of immune-related genes (IFN-γ: 1.96-fold; IL-10: 2.06-fold; IL-12: 1.91-fold; IL-1β: 4.07-fold; CYP-1α: 2.07-fold) and elevated oxidative stress markers (SOD: 2.96-fold; CAT: 3.52-fold; GSH: 3.07-fold; TAC: 2.58-fold) compared to uninfected controls (p < 0.0001 for all comparisons). Similarl

SUBMITTER: Salem MA 

PROVIDER: S-EPMC12569213 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature