Folic Acid Supplementation Attenuates Hepatic Steatosis by Enhancing Choline Availability and Remodeling Fatty Acid Profiles in Mice Fed a High-Fat Diet.

ABSTRACT: High-fat diets (HFDs) are a well-established cause of hepatic steatosis, a condition associated with altered hepatic fatty acid metabolism and reduced choline availability. Folic acid (FA) deficiency can also promote steatosis, in part by impairing choline metabolism. Although FA supplementation has been found to reduce liver fat in mice with hepatic steatosis, it is unclear if this effect is due to increased hepatic choline levels, changes in fatty acid profiles, or a combination of both. In this study, four-week-old male C57BL/6J mice were fed 45 kcal% HFDs with total FA content adjusted to onefold, fivefold, or tenfold AIN-93G recommended level (2 mg/kg diet) for 15 weeks ad libitum. Hepatic triacylglycerol (TAG), choline concentrations, expression of key genes in choline metabolism, an

SUBMITTER: Kranenburg E 

PROVIDER: S-EPMC12569376 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature