Evaluation of Measles Vaccine Immunogenicity and Durability Using A Pseudotyped Virus Neutralization Assay.

ABSTRACT:

Introduction

This study aimed to establish a robust method for monitoring measles vaccine-induced immunity and assessing population-level serostatus.

Methods

This study constructed a vesicular stomatitis virus (VSV)-based pseudotyped virus system expressing envelope proteins from seven major circulating measles genotypes (H1, B3, D4, D8, D9, D11, G3) and the Schwarz vaccine strain (genotype A), thereby enabling a high-throughput neutralization assay for antibody detection.

Results

Vaccination induced a substantial increase in neutralizing antibody geometric mean titers (GMT) post-immunization (4,808 after the first dose; 5,326 after the second dose), with antibody levels remaining elevated in 4-year-old children (GMT: 3,834). Cross-neutralization activity against diff

SUBMITTER: Jiang Q 

PROVIDER: S-EPMC12569535 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature