Algorithm-Assisted Structure Identification of Individual Nucleobases in Single DNA Chains through Tip-Enhanced Raman Spectromicroscopy.

ABSTRACT: The functions of biomolecules such as DNA are not only determined by their sequences but also strongly dependent on their stereostructures and geometric configurations of constituent units. However, most DNA sequencing techniques developed to date heavily rely on fluorescent labeling and extensive replication, and the DNA chains might thus be unwound and even modified during these treatments, resulting in the loss of structural information. Recent development of single-molecule tip-enhanced Raman spectroscopy (TERS) offers a label-free approach to identifying individual nucleobases with both high spatial resolution and chemical sensitivity but amounts to mainly proof-of-principle demonstrations of very short single-stranded DNA molecules. Here, we propose an algorithm-assisted strategy for

SUBMITTER: Zhu LY 

PROVIDER: S-EPMC12569700 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature