A modular scaffold for cellularly-retained fluorogenic probes for sensitive cell-resolved bioactivity imaging.

ABSTRACT: Here, we develop a general design for high-quality fluorogenic activity probes to quantify biochemical processes within live cells, via the release of a fully cell-retained, bright fluorescent soluble product upon reaction. Live cell probes must be membrane-permeable to access intracellular biochemistry, but often that means their fluorophore products are similarly permeable resulting in rapid signal loss from the activating cell, which limits their cell-by-cell resolution as well as their sensitivity for quantifying low-turnover processes. Current strategies to retain fluorescent products within cells usually disrupt native biology e.g. by non-specific alkylation or solid precipitation. Here, scanning charge- and polarity-based approaches to swap from permeable to cell-retai

SUBMITTER: Mauker P 

PROVIDER: S-EPMC12569792 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature