Serial enrichment incubation technique (SEIT) for rapid isolation of plant growth-promoting bacteria: Saving researcher time and lab resources.

ABSTRACT: Traditional methods of Plant growth-promoting bacteria (PGPB) isolation involve serial dilution and spreading on common or specific media, which leads to more than 10 bacteria per sample, and screening of these bacteria for a desired potential bacterium takes much time (4-6 months) and lab resources. The current improved protocol, termed Serial Enrichment Incubation Technique (SEIT), involves incubating the sample in a specific nutrient medium for 5 days, then transferring it into a fresh medium twice for five days each, and finally serially diluting the final tube and spreading the suspension onto Petriplates containing specific media. This protocol gives the desired nutrient-transforming bacteria compared to traditional published protocols within 20 days. Isolation of the siderophore-pro

SUBMITTER: Maheshwari HS 

PROVIDER: S-EPMC12569831 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature