Highly Sensitive Spatial Proteomics with Multicolor Cleavable Fluorescent Tyramide.

ABSTRACT: High-resolution single-cell spatial proteomics offers transformative insights into cellular diversity, architecture, interactions, and functions within complex biological systems. However, the existing multiplexed protein imaging platforms face challenges such as limited detection sensitivity, constrained target multiplexing capacity, or technically demanding. To address these issues, we report a highly sensitive spatial proteomics approach, using multicolor cleavable fluorescent tyramide and off-the-shelf antibodies. This method employs horseradish peroxidase (HRP) to enzymatically deposit distinct fluorophores to stain varied target proteins. Through reiterative cycles of target labeling, fluorescence imaging, and fluorophore cleavage, this approach allows numerous proteins profiled at t

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12569955 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature