High-throughput bioprinting to produce micropatterned neuroepithelial tissues and model TSC2-deficient brain malformations.

ABSTRACT: In vitro human pluripotent stem cell (hPSC)-derived models have been crucial in advancing our understanding of the mechanisms underlying neurodevelopment, though knowledge of the earliest stages of brain formation is lacking. Micropatterning of cell populations as they transition from pluripotency through the process of neurulation can produce self-assembled neuroepithelial tissues (NETs) with precise spatiotemporal control, enhancing the fidelity of hPSC models to the early developing human brain and their use in phenotypic assessments. Here, we introduce an accessible, customizable, and scalable method to produce self-assembled NETs using bioprinting to rapidly deposit reproducibly sized extracellular matrix droplets. Matrix addition to the media provides a scaffold that promotes 3D tiss

SUBMITTER: Imani Farahani N 

PROVIDER: S-EPMC12570318 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature