A real-time all-optical interface for dynamic perturbation of neural activity during behavior.

ABSTRACT: We developed a strategy for implementing a dream experiment in systems neuroscience, where circuit manipulation is guided by the real-time readout of neural activity in behaving mice. The system integrates a state-of-the-art calcium imaging analysis package that achieves rapid online activity readout from two-photon calcium imaging, a custom hologram generation program that targets two-photon optogenetic stimulation of specific neuronal ensembles, and software modules that automate essential steps in running complex all-optical experiments. Proof-of-principle experiments demonstrate that neurons can be automatically detected and recruited into a photostimulation ensemble, closed-loop photoinhibition can be implemented immediately after fast mapping of the functional properties of cortical

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12570328 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature