Rapid differentiation of viable and inactivated African swine fever virus by a viability quantitative PCR.

ABSTRACT: Real-time quantitative PCR (qPCR) has been widely used for African swine fever virus (ASFV) surveillance, but it cannot differentiate viable from inactivated virions, as inactivated particles often retain intact nucleic acids. This limitation may lead to overestimation of the presence of viruses in tested samples, potentially resulting in unnecessary outbreak responses and increased economic burdens. To address this challenge, a viability qPCR (V-qPCR) assay was established, utilizing nucleic acid intercalating dyes-propidium monoazide (PMA) or ethidium monoazide (EMA), to discriminate viable and inactivated ASFV. These dyes selectively penetrate compromised viral envelopes in inactivated particles, covalently binding viral DNA, and inhibiting PCR amplification. The optimized V-qPCR assay

SUBMITTER: Fan G 

PROVIDER: S-EPMC12570408 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature