Targeting METTL16 attenuates mesangial cell viability and fibrosis in a high-glucose state by suppressing m6A modification and the expression of RAP1B.

ABSTRACT:

Background

Methyltransferase-like 16 (METTL16) is involved in regulating kidney disease progression. This study aimed to investigate the effect of METTL16 on the progression of diabetic kidney disease (DKD) and its potential mechanism.

Methods

SV40-MES13 cells were transfected with METTL16 siRNA (siMETTL16), scramble control (siNC), or RAP1B siRNA (siRAP1B) under low glucose (LG) or high glucose (HG) conditions. Subsequently, RNA immunoprecipitation (RIP) assays, cell viability assays, EdU staining, TUNEL staining, ROS staining, and MDA detection were carried out.

Results

In SV40-MES13 cells, cell viability, the number of EdU-positive cells, the MDA level, METTL16 mRNA, and protein levels were greater in the HG groups than in the control group. Moreover, cell viabilit

SUBMITTER: Wang W 

PROVIDER: S-EPMC12570422 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature