Creating unity: linking 16S rRNA gene sequence information to the core taxonomy of genomes.

ABSTRACT:

Background

The Genome Taxonomy Database (GTDB) initiative aims to modernize prokaryotic taxonomy by aligning it with the great amounts of full-length genomes available today. Unfortunately, there is a poor link between the GTDB and the historically widely used 16S rRNA gene-based taxonomy. The current study explores the within and between divergence of the 16S rRNA gene sequences under GTDB taxonomy, refining our understanding of the 16S gene's resolution under this new taxonomic system. The analysis focuses on the divergence of 16S sequences collected from the GTDB genomes to identify optimal clustering thresholds for taxonomic resolution. Generalized linear mixed models (GLMMs) were fitted to estimate divergences within taxonomic ranks, correcting for the variable quality of the

SUBMITTER: Vinje H 

PROVIDER: S-EPMC12570451 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature