Single-cell landscape of mouse lungs exposed to intermittent hypoxia.

ABSTRACT:

Background

Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a prevalent respiratory disorder characterized by intermittent hypoxia (IH), which promotes pulmonary complications. However, the cellular and molecular mechanisms underlying IH-induced lung remodeling remain poorly understood.

Methods

We performed comprehensive single-cell RNA sequencing (scRNA-seq) analysis of lung tissue from IH-exposed mice (GSE145435). Computational approaches were used to characterize cellular heterogeneity, transcriptional programs, and cell-cell interactions. Key findings were validated using intervention studies with the SP1 inhibitor plicamycin.

Results

Our analysis revealed the following: IH induced: (1) the expansion of four distinct fibroblast subsets; (2) polarization of pro

SUBMITTER: Zheng P 

PROVIDER: S-EPMC12570456 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature