The resilience of <i>Salmonella</i> to bile stress is impaired due to the reduced efflux pump activity mediated by the antioxidant enzyme YqhD.

ABSTRACT: Bile salts play a critical role in modulating the host gut. They possess antimicrobial properties wherein they disrupt the bacterial membrane and produce reactive oxygen species (ROS), causing DNA damage. Pathogens like Salmonella regulate their metabolic activity to counteract the effects of bile. This study investigates the role of YqhD, an aldehyde reductase, in Salmonella's bile salt susceptibility. Our findings reveal increased survival of the yqhD mutant in the in-vitro studies in LB media with bile, liver cell line HepG2 and C57BL/6 mice on treatment with 8% sodium cholate in the cecum. Bile salts, physiologically produced for the digestion of fat, enhanced the organ burden of the yqhD mutant in C57BL/6 mice on replacing the chow diet with a high-f

SUBMITTER: Parmar K 

PROVIDER: S-EPMC12570503 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature