Microgravity-driven Rab27B activation amplifies mesenchymal stem cell-derived extracellular vesicle production and functions.

ABSTRACT:

Background

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) are emerging as promising treatments for immunomodulation and tissue regeneration. However, the scalable production of functionally enhanced EVs remains a critical challenge. This study introduces microgravity culture using a three-dimensional (3D) rotating cell culture system as a novel strategy to optimize MSC-EVs yield and bioactivity.

Methods

We first investigated the effects of microgravity on the proliferation and stemness of human umbilical cord-derived MSCs (UCMSCs). The yield of microgravity-derived EVs (µg-EVs) was quantified by nanoparticle tracking analysis. The function of µg-EVs was analyzed by proteomic profiling and further assessed by macrophage polarization and osteogenic differentiat

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12570638 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature