Macrophage-derived exosomes carrying miR-30a-5p alleviates IL-13/IL4-induced epithelial-mesenchymal transition in mouse bronchial epithelial cells via Runx1.

ABSTRACT:

Background

Allergic asthma (AA) has an increasing incidence rate. Macrophage-derived exosomes (EXO) carrying microRNAs (miRNAs) regulate epithelial-mesenchymal transition (EMT) in AA, which is worth further exploration. MiR-30a-5p is a small molecule with the potential to inhibit EMT.

Methods

To explore miR-30a-5p's effects on EMT, this study established OVA-induced AA mice, evaluating the model through airway hyperresponsiveness, immune cell counts in bronchoalveolar lavage fluid, Masson staining, and Western blot. Using quantitative real-time polymerase chain reaction and fluorescence in situ hybridization assays observed gene levels. Then mouse alveolar macrophages (MH-S) were co-cultured with primary mouse bronchial epithelial cells (BECs), incubated with IL-13 (10 ng/mL

SUBMITTER: Wu B 

PROVIDER: S-EPMC12570689 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature