Optimizing LED photobiomodulation parameters to prevent cartilage matrix degradation in knee osteoarthritis: in vitro and in vivo study.

ABSTRACT:

Background

Photobiomodulation using light-emitting diode (LED) therapy is a promising and safe non-invasive approach for treating knee osteoarthritis (KOA), yet the most effective wavelength and energy density to prevent cartilage matrix degradation remain unclear. This study aimed to determine the optimal LED parameters for suppressing chondrocyte extracellular matrix (ECM) degradation in vitro, and for alleviating ECM degradation and pain-related behaviours in a murine KOA model.

Methods

TNF-α-stimulated RCJ 3.1.C5.18 chondrocytes were irradiated with LEDs (625/810/940/1050 nm; 13-78 J/cm² at 44 mW/cm²). Chondrocyte ECM-related markers (mmp3, mmp13, col2a1, aggrecan) were quantified via RT-qPCR. In vivo, destabilization of the medial meniscus (DMM)-in

SUBMITTER: Fan T 

PROVIDER: S-EPMC12570694 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature