A framework for identifying and prioritizing SNPs in genes of the hypothalamic- pituitary-gonadal axis in Guzerat cattle using amplicon-based NGS.

ABSTRACT: The combined use of NGS technologies with bioinformatics tools has significantly advanced research by enabling comprehensive analyses of entire genomes, specific genomic regions of interest, and transcriptomes. Targeted NGS methods, which focus on smaller genome fractions, are widely used to study genetic diseases, epigenetic modifications, microbiomes, and environmental DNA, among other applications. This study aimed to develop a roadmap for detecting and selecting polymorphisms in candidate genes by integrating amplicon NGS-Target techniques with bioinformatics analyses. Sixty-eight genes associated with the hypothalamic-pituitary-gonadal (HPG) axis were selected to develop the amplicon NGS assay, comprising 730 regions that cover a total of 136,274 bp. This method was used to sequence 7

SUBMITTER: Marcuzzi O 

PROVIDER: S-EPMC12570696 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature