Heterogeneous origins and functions of lung mesenchymal stromal cells.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) and their secretomes have been extensively studied for the treatment of various pulmonary diseases; however, consistent therapeutic benefits have yet to be demonstrated. The fundamental biology of lung-resident MSCs remains poorly understood.

Methods

We used a previously characterized fetal lung mesenchyme-specific reporter mouse model to initially identify lung MSC subpopulations based on their developmental origins. Bulk RNA-seq was then used to confirm mouse lung MSC differences at the transcriptomic level and to separate human lung MSC subsets. A variety of in vitro methods were used to measure cell proliferation, differentiation, adhesion, T cell modulation, alveolar epithelial cell growth.

Results

We identified two dis

SUBMITTER: Peng D 

PROVIDER: S-EPMC12570732 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature