NSUN2 promotes leukemogenesis by ferritin heavy chain 1-mediated anti-ferroptosis and enhancing global protein synthesis in an m⁵C-dependent manner in B-acute lymphoblastic leukemia.

ABSTRACT:

Background

NOP2/Sun RNA methyltransferase family member 2 (NSUN2) catalyzes 5-methylcytosine (m⁵C) modifications in tRNA, rRNA, and mRNA, playing critical roles in various malignancies. However, its expression and functional relevance in B-acute lymphoblastic leukemia (B-ALL) remain largely undefined.

Methods

Lineage-negative progenitor cells from Nsun2 knockout (Δ/Δ) and wild-type (fl/fl) mice were used to generate BCR-ABL (P190)-driven murine B-ALL model. Furthermore, RNA sequencing (RNA-seq) was performed to identify NSUN2 targets. Cell proliferation, apoptosis, and colony formation, and global protein synthesis were measured in B-ALL cells with NSUN2 deficiency.

Results

Here, we found that NSUN2 expression was significantly higher in bone marrow (BM) ce

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC12570736 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature