Dynamic molecular and cellular characteristics of VSX2-positive retinal progenitor cells in human retinal organoids.

ABSTRACT:

Background

The lack of understanding of the molecular and cellular characteristics of human retinal progenitor cells (RPCs) has hindered their application in cell therapy for retinal degenerative diseases. This study aims to employ retinal organoids (ROs) derived from a VSX2-enhanced green fluorescent protein (eGFP) reporter human induced pluripotent stem cell (hiPSC) line for positive selection of human RPCs, investigate their features, and facilitate their applications.

Methods

hiPSCs were differentiated into three-dimensional ROs following established protocols. The fidelity of the VSX2-eGFP reporter was confirmed through immunostaining. Fluorescence-activated cell sorting was employed to select VSX2-eGFP-positive (+) cells at distinct developmental stages, followed by bu

SUBMITTER: Zheng D 

PROVIDER: S-EPMC12570740 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature