In vitro biomimetic development of artificial dental calculus: a proof of concept study.

ABSTRACT:

Background

To develop an in vitro model to generate dental calculus through a standardized process of biofilm mineralization, and compare it with natural human dental calculus .

Methods

The proposed in vitro model consisted of two staged procedures: first biofilm formation and then its mineralization. After a 72-hour pre-culture, biofilms of Streptococcus mutans were formed on a glass dish in artificial saliva with 1% sucrose for 3 h. Then the calcification process consisted of incubating the sample in supersaturated mineral solution for 21 h. These steps were repeated for 5 days. The samples were then divided into 3 groups based on the different processes; (i) mineralization only, (ii) biofilm formation only, (iii) biofilm formation and mineralization. Obtained samples were

SUBMITTER: Cha JK 

PROVIDER: S-EPMC12570754 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature