Effect of Depletion of Developmentally Regulated GTP Binding Protein on Osteoblastic Differentiation and Bone Microarchitecture.

ABSTRACT: Previous studies have demonstrated that overexpression of DRG2 enhances the osteoclastic activity. However, its impact on osteoblastic differentiation remains unexplored. This study investigates the relationship between DRG2 and osteoblastic activity through in vitro and in vivo studies. DRG2 expression was inhibited in mouse MC3T3-E1 cells, and its effects on the expression of bone formation markers and osteoblast-related transcription factors were evaluated. The capacity for osteoblastic differentiation was assessed in mouse preosteoblasts following DRG2 gene knockdown using a short hairpin RNA (shRNA) plasmid. Female C57BL/6N strain DRG2 knockout (KO) mice were generated, and bone phenotypes of the vertebrae and femurs were analysed. Additionally, osteoblastic differentiation capacity w

SUBMITTER: Jin YZ 

PROVIDER: S-EPMC12571191 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature