Tracker Nanocatalyst for Screening of Intracellular Copper-Catalyzed Azide-Alkyne Cycloadditions.

ABSTRACT: Intracellular copper-catalyzed azide-alkyne cycloaddition (CuAAC) offers immense potential for bioorthogonal chemistry, but its application is severely hindered by copper toxicity and the challenge of controlling catalysis within the complex cellular environment. Heterogeneous copper catalysts can reduce toxicity by minimizing free copper exposure and enabling localized activity, yet optimizing their performance in situ within living cells remains a significant hurdle. Here, the development of a novel dual-functional nanocatalyst, Cu@BTTAA-Cy5-NPs, that combines robust heterogeneous CuAAC catalytic activity with intrinsic fluorescence tracking is reported. The successful synthesis and characterization of these monodispersed nanoparticles is demonstrated, confirming efficient copper loading

SUBMITTER: Rodriguez-Segura M 

PROVIDER: S-EPMC12571216 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature