ATF3 prevents retinal ganglion cell apoptosis and mitigates microglia-mediated neuroinflammation in retinal ischemia-reperfusion injury.

ABSTRACT:

Introduction

Neuroinflammation is a key pathological response involved in secondary optic nerve injury following retinal ischemia-reperfusion injury. The expression of activating transcription factor 3 (ATF3), a highly conserved protein, is rapidly induced post-injury and is crucial for regulating immunity and inflammation. The potential neuroprotective mechanisms, function, and therapeutic potential of ATF3 following retinal ischemia-reperfusion remain largely unexplored. In this study, we examined the expression and distribution of ATF3 and achieved the overexpression of ATF3 in mouse retina via injection of adeno-associated virus vectors.

Methods

Retinal ganglion cell survival was assessed using immunofluorescence staining and terminal deoxynucleotidyl transferase dUTP ni

SUBMITTER: Zhang N 

PROVIDER: S-EPMC12571733 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature