LpxR and PagL expression in live attenuated auxotrophic <i>Pseudomonas aeruginosa</i> vaccines modulates lipid A reactogenicity <i>in vitro</i> while preserving immunogenicity.

ABSTRACT:

Introduction

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen responsible for severe respiratory tract infections. We previously developed a live attenuated auxotrophic vaccine candidate, PAO1 ΔmurI, which conferred protection in murine infection models but exhibited significant reactogenicity when administered intranasally. To reduce the toxicity of PAO1 ΔmurI without compromising its protective efficacy, we engineered strains with a modified lipid A structure, as lipid A is one of the main toxic components of whole-cell vaccines.

Methods

Two lipid A-modifying enzymes, LpxR and PagL were overproduced in PAO1 ΔmurI derivatives. The resulting lipopolysaccharide (LPS) was analyzed by MALDI-TOF mass spectrometry. In vitro assay

SUBMITTER: Moscoso M 

PROVIDER: S-EPMC12571757 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature