Cold stress adaptation in <i>Trifolium ambiguum</i>: physiological and transcriptomic insights.

ABSTRACT:

Introduction

Cold stress significantly affects the growth and distribution of leguminous forage crops. Trifolium ambiguum, a cold-tolerant perennial clover, exhibits distinct physiological and molecular responses to cold stress. Understanding its cold adaptation mechanisms is crucial for improving the resilience of forage crops.

Methods

In this study, we analyzed the physiological and transcriptomic responses of T. ambiguum seedlings exposed to 0°C for 0, 2, 6, and 12 hours. Various physiological parameters, including antioxidant enzyme activities (SOD, POD, and CAT), proline, and soluble sugar levels, were measured. Transcriptomic analysis was performed to identify the gene expression changes associated with cold stress.

Results

The results indicated th

SUBMITTER: Cao K 

PROVIDER: S-EPMC12571821 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature