Isolation, functional characterization and antibiofilm properties of a lytic Enterococcus phage RG1 against multidrug resistant E. faecium.

ABSTRACT: Enterococcus faecium, a multidrug-resistant (MDR), commensal human pathogen, frequently causes nosocomial infections and imposes serious threat to public health, which demanded more research for the development of alternative therapeutics against them. Bacteriophage therapy as an alternative to antibiotics has reappeared as therapeutics against MDR bacterial infections. Here, we isolated and characterized a novel lytic phage, RG1, from the Ganga River against E. faecium ATCC 35667 and checked their efficacy against several clinical isolates of E. faecium. Whole genome sequencing revealed that the RG1 belongs to genus Efemquintavirus, and has a dsDNA genome of 41,364 base pairs with 35.54% GC content, which encodes 65 putative open reading frames without any virulence, antibiotic resistance

SUBMITTER: Singh RK 

PROVIDER: S-EPMC12572221 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature