Real-time PCR assay design and validation for Prevotella bivia, Peptostreptococcus anaerobius, and Dialister micraerophilus, bacteria associated with increased HIV susceptibility.

ABSTRACT: Many genital anaerobes, including Prevotella bivia, Peptostreptococcus anaerobius, and Dialister micraerophilus, are underexplored despite being Bacteria Associated with HIV Seroconversion, Inflammation, and immune Cells (BASICs). This is partly due to the lack of molecular tools for their detection and quantification. To address this gap, we designed and validated three real-time qPCR assays for rapid and cost-effective analysis. qPCR assays were designed based on the species-specific core genomes of the three target genital anaerobic species. Assay sensitivity, specificity, and quantification characteristics were assessed using a synthetic oligonucleotide and DNA extracted from closely related species (n = 27-42) and human urogenital swabs (n = 111-114). The resultant assays demonstrated

SUBMITTER: Nelson SG 

PROVIDER: S-EPMC12572226 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature