Proteome of seminal plasma and sperm associated with sperm survival following cryopreservation in the Red Wolf (Canis rufus).

ABSTRACT: Understanding of the impacts of seminal plasma and sperm proteins on both animal and human fertility has expanded rapidly in the last decade. However, little is known about the reproductive proteomes of endangered species. In this study, we characterized for the first time the seminal plasma and sperm proteomes of the critically endangered Red Wolf and evaluated differences in proteomic signatures between ejaculates which experienced "High" versus "Baseline" (i.e. population average) sperm cryo-resilience. Highly cryo-resilient ejaculates (High_CR, n = 5) maintained > 50% sperm total motility relative to fresh samples following cryopreservation and post-thaw incubation, as well as acrosome integrity post-thaw. These samples had significantly higher expression of A1BG, APBB1, KRT1, KRT10, L

SUBMITTER: Celedon JJ 

PROVIDER: S-EPMC12572227 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature