Plasma-activated medium induces apoptosis of oral squamous cell carcinoma through the MAPKs pathway.

ABSTRACT: No-ozone cold plasma (NCP) is actively being researched as an alternative to address various side effects of cancer treatment. Compared to traditional direct procedures, the indirect method using plasma-activated medium (PAM) has advantages like greater tissue penetration and convenience. The purpose of this study is to assess the efficacy of plasma-activated medium on oral squamous cell carcinoma both in vitro and in vivo. Squamous Cell Carcinoma cells (SCC-25) and human keratinocyte cells (HaCaT) were used in this experiment. The evaluation of cell viability was conducted through sulforhodamine B (SRB) analysis. The effect of PAM was observed on the cytoskeletal structure through F-actin staining. To analyze cell selectivity, immunofluorescence staining was conducted to monitor alteratio

SUBMITTER: Jang H 

PROVIDER: S-EPMC12572278 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature