In vitro approaches to study centriole and cilium function in early mouse embryogenesis.

ABSTRACT: Although centrioles and primary cilia play an essential role in early mammalian development, their specific function during the interval between their initial formation and the subsequent arrest of embryogenesis in embryos deficient in centrioles or cilia remains largely unexplored. Here, we demonstrate that different 3D in vitro model systems recapitulate early centriole and cilium formation in mouse development. Centrioles and cilia are dispensable in 3D in vitro mouse rosettes, a model system that mimics key events of implantation, including polarization and lumenogenesis. In gastruloids, a model system that recapitulates developmental processes up to E8.5, centriole loss results in early disassembly that can be rescued by additional p53 deletion. In contrast, cells devoid of cilia cont

SUBMITTER: Voelkl I 

PROVIDER: S-EPMC12572739 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature