Implant Renal Injury-Responsive Cells to Supplement Erythropoietin and Protect Kidney Injury.

ABSTRACT: Anemia poses a life-threatening risk to individuals with chronic kidney diseases (CKDs). Insufficient production of erythropoietin (EPO) from the injured kidney is the major reason for anemia, while lack of EPO would further aggravate the kidney injury and make a "vicious cycle." In this study, we dissected the change of endogenous EPO signaling in the injured kidney by spatial transcriptomic analysis and validated the dual beneficial effects of local recombinant EPO administration on both anemia correction and kidney protection. Next, we constructed an injury-responsive EPO-producing (iREP) element to sense the kidney damage signal and drive the synthesis and secretion of native EPO. After intrarenal implantation of iREP-engineered HEK-293T embryonic kidney cells, the mouse kidney would a

SUBMITTER: Nie H 

PROVIDER: S-EPMC12572954 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature