Comprehensive Proteomics Profiling Reveals Biomarkers of Oxidative Stress and Membrane Integrity in Red Blood Cells in Acute Altitude Sickness.

ABSTRACT: Red blood cells (RBCs) are vital for oxygen transport and play a key role in acute mountain sickness (AMS). Phosphorylation is one of the most rapid post-translational modifications in response to environmental changes. However, the relationship between the changes of phosphoproteins in the RBC membrane and AMS has not received much attention. We conducted proteomic and phosphoproteomic profiling of RBC (including reticulocytes and mature erythrocytes) membranes from AMS and non-AMS individuals, identifying 2383 proteins, 1076 phosphoproteins, and 2913 phosphopeptides. Specifically, AMS caused significant content changes in the proteins and phosphorylation events of the tricarboxylic acid cycle, cytoskeleton, and peroxisome. We further determined the membrane proteins and phosphopeptides r

SUBMITTER: Li S 

PROVIDER: S-EPMC12572991 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature