Aptamer-conjugated core-shell magnetic nanoparticles for quick and specific separation of gram-negative bacteria.

ABSTRACT: Quick separation of gram-negative bacteria, such as Escherichia coli (E. coli), from water sources is crucial for ensuring timely public health protection and promoting environmental sustainability. This study demonstrates that aptamer-conjugated core-shell magnetic nanoparticles (MNPs) can quickly capture E. coli DH5α from water. The core-shell MNP composed of an iron (Fe₃O₄) core and a silica (SiO₂) shell has been bio-conjugated with aptamers using glutaraldehyde as a cross-linker. The Fe₃O₄ core has an average diameter of approximately 16 ± 5 nm, and the thickness of the silica shell is around 24 ± 4 nm. Our results show that 1 mg mL^-1 of the Aptamer-Fe₃O₄@SiO₂ nanopar

SUBMITTER: Panta R 

PROVIDER: S-EPMC12573078 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature