Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics.

ABSTRACT: Versatile technologies that can deliver both RNA and protein payloads could streamline development, simplify manufacturing and expand the capabilities of combination therapies. Here we demonstrate an efficient approach to forming ca. 100 nm polymer vesicles (polymersomes) capable of rapid self-assembly without organic solvents, avoiding the need for post-encapsulation purification. Block copolymers are designed with a lower critical solution temperature that renders them soluble in aqueous medium under standard refrigeration, but they spontaneously assemble at room temperature into large batches of nanoparticles with predictable size and morphology. The nanomaterials are designed with charged and biofunctional moieties to drive payload affinity and in vivo targeting, while both siRNA and p

SUBMITTER: Hossainy S 

PROVIDER: S-EPMC12573146 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature