Development of an Ultrasensitive Monoclonal Antibody against Butralin Based on a Novel Hapten Design Strategy.

ABSTRACT: Butralin (BL) is a dinitroaniline herbicide commonly applied in agriculture to control annual grasses and broadleaf weeds. However, its environmental persistence and potential toxicity to nontarget organisms, including aquatic species and humans, have raised serious concerns. Reliable monitoring of BL residues is therefore crucial for ensuring environmental and food safety. In this study, a novel hapten was synthesized by introducing an ethylenediamine group at the C1 position of 4-tert-butyl-2,6-dinitrobenzene, followed by spacer arm extension via succinic anhydride acylation. Using this hapten, a specific monoclonal antibody (mAb) against BL was generated, exhibiting the highest affinity reported to date. Based on this mAb, an optimized indirect competitive ELISA (ic-ELISA) was es

SUBMITTER: Li YJ 

PROVIDER: S-EPMC12573165 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature