A Universal Spectrum Annotator for Complex Peptidoforms in Mass Spectrometry-Based Proteomics.

ABSTRACT: Accurate and comprehensive peptide spectrum annotation is a crucial step to interpreting mass spectrometry-based proteomics data. While peak assignment in peptide fragmentation spectra is central to a broad range of proteomics applications, current tools tend to be specialized to a specific task. Here, we present a more comprehensive interactive graphical tool (Annotator), along with the underlying codebase written in Rust (rustyms). Annotator enables unified spectrum annotation for bottom-up, middle-down, top-down, cross-linked, and glycopeptide fragmentation mass spectra from all fragmentation methods, including all ion types: a/b/c, x/y/z, d/v/w, and immonium ions. The Annotator integrates all known post-translational modifications from common databases and additionally allows for the d

SUBMITTER: Schulte D 

PROVIDER: S-EPMC12573231 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature