LRRC8A in endothelial cells contributes to the aberrant blood-brain barrier integrity in ischaemic stroke.

ABSTRACT:

Background

The increased permeability of the blood-brain barrier (BBB) is a critical contributor to the high mortality following ischaemic stroke. However, the mechanisms regulating BBB integrity remain poorly understood. Leucine-rich repeat-containing 8A (LRRC8A) is a chloride channel critical for cellular volume homeostasis and plays a key role in regulating neuronal injury during ischaemia. However, its impact on BBB function is currently unclear.

Methods

A transient middle cerebral artery occlusion model was established to investigate the impact of LRRC8A on BBB integrity. Laser speckle contrast imaging was used to monitor cortical blood flow. Primary mouse and human brain microvascular endothelial cells (m/hBMVECs) were subjected to oxygen-glucose deprivation (OGD) and

SUBMITTER: Tian Y 

PROVIDER: S-EPMC12573346 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature