Improved rapid and efficient hairy root transformation using <i>Rhizobium rhizogenes</i> in legume crops.

ABSTRACT: Hairy root transformation mediated by Rhizobium rhizogenes is a widely used tool for molecular analysis and root material for secondary metabolite production. However, this method is time-intensive, technically demanding, and exhibits low transformation efficiency. To address these limitations, we developed a rapid and efficient hairy root transformation system for legume crops, optimizing protocols with the soybean (Glycine max L. Merrill) cultivar Fukuyutaka. Sterilizing seeds with vapor of 5% sodium hypochlorite and germinating them in a double-tier container resulted in over 90% healthy, straight seedlings ideal for transformation, with 3- to 5-day-old seedlings showing the highest transformation rates. Exposing the plant shoot during co-cultivation by covering only the i

SUBMITTER: Araragi M 

PROVIDER: S-EPMC12573540 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature