Single-cell Raman spectroscopic analysis of bacteroids in soybean nodules to observe the relationship between biomolecular constituents and symbiotic nitrogen fixation activity.

ABSTRACT: Nitrogen fixation in soybean occurs as a result of symbiosis between the plant and rhizobia in the nodules. This process allows both the plant and the symbiont to acquire vital nutrition. To fully understand the symbiosis, many researchers have attempted to attain a deeper interpretation of the biomolecular behavior or enhance the nitrogen fixation activity of bacteroids. However, most studies have focused on forward and reverse genetics approaches to evaluate the contribution of a particular gene/enzyme in nitrogen fixation. Few studies have observed the bacteroids' overall biomolecular behavior in the nodules. Thus, we grew soybean plants and recorded acetylene reduction assay (ARA) results at several growth stages. Simultaneously, we analyzed the biomolecular compounds in the bacteroids

SUBMITTER: Suwa S 

PROVIDER: S-EPMC12573639 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature