Towards synthetic catechol rich protein analogues through tyrosinase catalyzed activation of a tyrosine dipeptide in continuous mode.

ABSTRACT: We present a perspective towards a green synthesis route for synthetic, catechol rich protein analogues (TCC). The method relies on the oxidation of a tyrosine dipeptide in continuous mode by the immobilized tyrosinase SinATyr followed by Michael addition of a dithiol. For the dipeptide substrate a k _cat value of 0.16 s^-1 and a K _m value of 1.6 mM were determined meaning that its conversion is slower and the affinity towards the active center of the enzyme is lower compared to the standard substrate l-tyrosine (k _cat = 5.6 s^-1; K _m = 0.24 mM). For the continuous operation mode SinATyr is immobilized on polyelectrolyte decorated silica microparticles with a k value of 0.11 s

SUBMITTER: Reinicke S 

PROVIDER: S-EPMC12573765 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature