A novel method for effectively selecting fragments not associated with restriction sites for whole-genome genotyping.

ABSTRACT:

Background

Reduced representation sequencing (RRS) using Restriction site-associated DNA sequencing (RAD-seq) has become a widely adopted method for whole-genome genotyping, owing to its cost-effectiveness and applicability across species. However, traditional RAD-seq approaches face challenges, including complex workflows and high labor demands. Existing RAD-seq methods rely primarily on the strategy of "selecting fragments first, then preparing a library," which involves targeting fragments associated with restriction enzyme cut sites.

Results

In contrast, we developed inverse RAD-seq (iRAD-seq), a novel method that employs a "prepare library first, then select" strategy to efficiently capture fragments not associated with restriction sites for whole-genome genotyping. Thi

SUBMITTER: Chen P 

PROVIDER: S-EPMC12573827 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature