Next-generation stress-inducible Komagataella phaffii promoter variants.

ABSTRACT:

Background

Expanding the promoter toolbox of Komagataella phaffii (K. phaffii) in terms of strength and regulatory flexibility can significantly enhance bioprocess efficiency for recombinant protein and metabolite production. The most frequently used promoters are still derived from the methanol utilization (MUT) pathway or genes of the central metabolism. However, the hazards and costs associated with methanol have prompted the search for alternative promoters, including engineered variants. A key limitation remains, many available promoters are still growth-coupled, tying production to biomass accumulation and shortening process duration. Promoters with growth-decoupled expression are therefore highly desirable. In this context, the recently described P_DH promoter is o

SUBMITTER: Ebner K 

PROVIDER: S-EPMC12573857 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature