Metabolic engineering of Streptomyces explomaris for increased production of the reverse antibiotic nybomycin.

ABSTRACT:

Background

Nybomycin is a reverse antibiotic with selective activity against fluoroquinolone-resistant Gram-positive bacteria, including Staphylococcus aureus, making it a promising candidate to fight against antimicrobial resistance. However, its clinical development has been limited by the low production yields of native producers. To address this, we explored the heterologous expression of the nybomycin biosynthetic gene cluster (nyb) from the natural producer S. albus subsp. chlorinus NRRL B-24,108 in different marine and terrestrial Streptomyces hosts, aiming to boost production through targeted regulatory and metabolic engineering. We also evaluated the use of seaweed-derived hydrolysates as sustainable fermentation substrates.

Results

Among several tested hosts, S. ex

SUBMITTER: Shu W 

PROVIDER: S-EPMC12573870 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature