`Mix-it-up': accessible time-resolved cryo-EM on the millisecond timescale.

ABSTRACT: Biological reactions often involve macromolecules that undergo substrate-induced conformational changes in under a second, yet capturing these transient states remains challenging. While high-resolution structural techniques such as X-ray crystallography and cryo-electron microscopy (cryo-EM) have advanced our mechanistic understanding of protein-substrate interactions, traditional sample-preparation methods are too slow to capture rapid biochemical events. Time-resolved cryo-EM has emerged as a promising approach to visualize structural dynamics on microsecond-to-millisecond timescales, but its widespread adoption has been limited by costly equipment and challenges in achieving rapid mixing, application and vitrification of samples in a reproducible manner. To address these limitations, w

SUBMITTER: Alexandrescu L 

PROVIDER: S-EPMC12573921 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature